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Extraction of chitosan and its oligomers from shrimp shell waste, their characterization and antimicrobial effect
AIM: The present study was performed to utilize the shrimp shell waste for chitin and chitosan production, characterization by Fourier transform infrared (FT-IR) technique and to evaluate the antimicrobial effects of chitosan oligomers produced by depolymerization of chitosan by nitrous acid. MATERI...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Veterinary World
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5352841/ https://www.ncbi.nlm.nih.gov/pubmed/28344399 http://dx.doi.org/10.14202/vetworld.2017.170-175 |
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author | Varun, Tarun Kumar Senani, Swaraj Jayapal, Natasha Chikkerur, Jayaram Roy, Sohini Tekulapally, Vijay Bhasker Gautam, Mayank Kumar, Narender |
author_facet | Varun, Tarun Kumar Senani, Swaraj Jayapal, Natasha Chikkerur, Jayaram Roy, Sohini Tekulapally, Vijay Bhasker Gautam, Mayank Kumar, Narender |
author_sort | Varun, Tarun Kumar |
collection | PubMed |
description | AIM: The present study was performed to utilize the shrimp shell waste for chitin and chitosan production, characterization by Fourier transform infrared (FT-IR) technique and to evaluate the antimicrobial effects of chitosan oligomers produced by depolymerization of chitosan by nitrous acid. MATERIALS AND METHODS: Chitosan was extracted from the shrimp shell waste by the chemical method and characterized by FT-IR. Chitooligomers were produced by depolymerising chitosan using nitrous acid, and the chitooligomers were tested for antimicrobial effect against four gut pathogenic organisms, i.e., Enterobacter aerogen (National Collection of Dairy Culture [NCDC] 106), Enterococcus faecalis (NCDC 119), Escherichia coli (NCDC 134), and Staphylococcus aureus (NCDC 109) by well diffusion method using Muller-Hinton agar. A pure culture of pathogenic organisms was collected from NCDC, ICAR-National Dairy Research Institute, Karnal. RESULTS: Extracted chitosan characterized by FT-IR and chitooligomers demonstrated antimicrobial effect against four gut pathogenic organisms used in this study. Zone of inhibitions (mm) were observed in E. faecalis (13±0.20), E. coli (11.5±0.4), S. aureus (10.7±0.2), and E. aerogen (10.7±0.3). E. faecalis showed larger inhibition zone as compared to all other organisms and inhibitions zones of E. aerogen and S. aureus were comparable to each other. CONCLUSION: Shrimp waste can be utilized for chitosan production, and the chitooligomers can be used as feed additive for gut health enhancement and have potential to replace antibiotics from the feed. Along with value addition pollutant load could be reduced by waste utilization. |
format | Online Article Text |
id | pubmed-5352841 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Veterinary World |
record_format | MEDLINE/PubMed |
spelling | pubmed-53528412017-03-24 Extraction of chitosan and its oligomers from shrimp shell waste, their characterization and antimicrobial effect Varun, Tarun Kumar Senani, Swaraj Jayapal, Natasha Chikkerur, Jayaram Roy, Sohini Tekulapally, Vijay Bhasker Gautam, Mayank Kumar, Narender Vet World Research Article AIM: The present study was performed to utilize the shrimp shell waste for chitin and chitosan production, characterization by Fourier transform infrared (FT-IR) technique and to evaluate the antimicrobial effects of chitosan oligomers produced by depolymerization of chitosan by nitrous acid. MATERIALS AND METHODS: Chitosan was extracted from the shrimp shell waste by the chemical method and characterized by FT-IR. Chitooligomers were produced by depolymerising chitosan using nitrous acid, and the chitooligomers were tested for antimicrobial effect against four gut pathogenic organisms, i.e., Enterobacter aerogen (National Collection of Dairy Culture [NCDC] 106), Enterococcus faecalis (NCDC 119), Escherichia coli (NCDC 134), and Staphylococcus aureus (NCDC 109) by well diffusion method using Muller-Hinton agar. A pure culture of pathogenic organisms was collected from NCDC, ICAR-National Dairy Research Institute, Karnal. RESULTS: Extracted chitosan characterized by FT-IR and chitooligomers demonstrated antimicrobial effect against four gut pathogenic organisms used in this study. Zone of inhibitions (mm) were observed in E. faecalis (13±0.20), E. coli (11.5±0.4), S. aureus (10.7±0.2), and E. aerogen (10.7±0.3). E. faecalis showed larger inhibition zone as compared to all other organisms and inhibitions zones of E. aerogen and S. aureus were comparable to each other. CONCLUSION: Shrimp waste can be utilized for chitosan production, and the chitooligomers can be used as feed additive for gut health enhancement and have potential to replace antibiotics from the feed. Along with value addition pollutant load could be reduced by waste utilization. Veterinary World 2017-02 2017-02-12 /pmc/articles/PMC5352841/ /pubmed/28344399 http://dx.doi.org/10.14202/vetworld.2017.170-175 Text en Copyright: © Varun, et al. http://creativecommons.org/licenses/by/4.0 Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Varun, Tarun Kumar Senani, Swaraj Jayapal, Natasha Chikkerur, Jayaram Roy, Sohini Tekulapally, Vijay Bhasker Gautam, Mayank Kumar, Narender Extraction of chitosan and its oligomers from shrimp shell waste, their characterization and antimicrobial effect |
title | Extraction of chitosan and its oligomers from shrimp shell waste, their characterization and antimicrobial effect |
title_full | Extraction of chitosan and its oligomers from shrimp shell waste, their characterization and antimicrobial effect |
title_fullStr | Extraction of chitosan and its oligomers from shrimp shell waste, their characterization and antimicrobial effect |
title_full_unstemmed | Extraction of chitosan and its oligomers from shrimp shell waste, their characterization and antimicrobial effect |
title_short | Extraction of chitosan and its oligomers from shrimp shell waste, their characterization and antimicrobial effect |
title_sort | extraction of chitosan and its oligomers from shrimp shell waste, their characterization and antimicrobial effect |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5352841/ https://www.ncbi.nlm.nih.gov/pubmed/28344399 http://dx.doi.org/10.14202/vetworld.2017.170-175 |
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