Increased dosage of AOX1 promoter-regulated expression cassettes leads to transcription attenuation of the methanol metabolism in Pichia pastoris

The methanol-regulated alcohol oxidase promoter (P(AOX1)) of Pichia pastoris is one of the strongest promoters for heterologous gene expression in this methylotrophic yeast. Although increasing gene dosage is one of the most common strategies to increase recombinant protein productivities, the incre...

Descripción completa

Detalles Bibliográficos
Autores principales: Cámara, Elena, Landes, Nils, Albiol, Joan, Gasser, Brigitte, Mattanovich, Diethard, Ferrer, Pau
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5353721/
https://www.ncbi.nlm.nih.gov/pubmed/28295011
http://dx.doi.org/10.1038/srep44302

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5353721/
https://www.ncbi.nlm.nih.gov/pubmed/28295011
http://dx.doi.org/10.1038/srep44302

Ejemplares similares