OEPR Cloning: an Efficient and Seamless Cloning Strategy for Large- and Multi-Fragments

Here, an efficient cloning strategy for large DNA fragments and for simultaneous assembly of multiple DNA fragments assembly is presented. This strategy is named OEPR (based on Overlap Extension PCR and Recombination in vivo). OEPR cloning is a seamless, restriction- and ligation-independent method....

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Detalles Bibliográficos
Autores principales: Liu, Chang-Jun, Jiang, Hui, Wu, Lei, Zhu, Ling-Yun, Meng, Er, Zhang, Dong-Yi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5353728/
https://www.ncbi.nlm.nih.gov/pubmed/28300166
http://dx.doi.org/10.1038/srep44648

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5353728/
https://www.ncbi.nlm.nih.gov/pubmed/28300166
http://dx.doi.org/10.1038/srep44648

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