Cargando…

Multiplex PCR as a tool for the diagnosis of Leishmania spp. kDNA and the gapdh housekeeping gene of mammal hosts

BACKGROUND: The PCR assays usually employed for Leishmania diagnosis does not simultaneously detect a constitutive gene that would certify the viability of the DNA sample. We present a multiplex PCR approach for the simultaneous diagnosis of the Leishmania sp. kDNA fragment and a catalytic domain se...

Descripción completa

Detalles Bibliográficos
Autores principales: de Cássia-Pires, Renata, de Melo, Myllena de Fátima Alheiros Dias, Barbosa, Raquel da Hora, Roque, André Luiz Rodrigues
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5354409/
https://www.ncbi.nlm.nih.gov/pubmed/28301553
http://dx.doi.org/10.1371/journal.pone.0173922