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DNA damage checkpoint adaptation genes are required for division of cells harbouring eroded telomeres

In budding yeast, telomerase and the Cdc13p protein are two key players acting to ensure telomere stability. In the absence of telomerase, cells eventually enter a growth arrest which only few can overcome via a conserved process; such cells are called survivors. Survivors rely on homologous recombi...

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Autores principales: Mersaoui, Sofiane Y., Gravel, Serge, Karpov, Victor, Wellinger, Raymund J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Shared Science Publishers OG 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5354583/
https://www.ncbi.nlm.nih.gov/pubmed/28357265
http://dx.doi.org/10.15698/mic2015.10.229
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author Mersaoui, Sofiane Y.
Gravel, Serge
Karpov, Victor
Wellinger, Raymund J.
author_facet Mersaoui, Sofiane Y.
Gravel, Serge
Karpov, Victor
Wellinger, Raymund J.
author_sort Mersaoui, Sofiane Y.
collection PubMed
description In budding yeast, telomerase and the Cdc13p protein are two key players acting to ensure telomere stability. In the absence of telomerase, cells eventually enter a growth arrest which only few can overcome via a conserved process; such cells are called survivors. Survivors rely on homologous recombination-dependent mechanisms for telomeric repeat addition. Previously, we showed that such survivor cells also manage to bypass the loss of the essential Cdc13p protein to give rise to Cdc13-independent (or cap-independent) strains. Here we show that Cdc13-independent cells grow with persistently recognized DNA damage, which does not however result in a checkpoint activation; thus no defect in cell cycle progression is detectable. The absence of checkpoint signalling rather is due to the accumulation of mutations in checkpoint genes such as RAD24 or MEC1. Importantly, our results also show that cells that have lost the ability to adapt to persistent DNA damage, also are very much impaired in generating cap-independent cells. Altogether, these results show that while the capping process can be flexible, it takes a very specific genetic setup to allow a change from canonical capping to alternative capping. We hypothesize that in the alternative capping mode, genome integrity mechanisms are abrogated, which could cause increased mutation frequencies. These results from yeast have clear parallels in transformed human cancer cells and offer deeper insights into processes operating in pre-cancerous human cells that harbour eroded telomeres.
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spelling pubmed-53545832017-03-29 DNA damage checkpoint adaptation genes are required for division of cells harbouring eroded telomeres Mersaoui, Sofiane Y. Gravel, Serge Karpov, Victor Wellinger, Raymund J. Microb Cell Microbiology In budding yeast, telomerase and the Cdc13p protein are two key players acting to ensure telomere stability. In the absence of telomerase, cells eventually enter a growth arrest which only few can overcome via a conserved process; such cells are called survivors. Survivors rely on homologous recombination-dependent mechanisms for telomeric repeat addition. Previously, we showed that such survivor cells also manage to bypass the loss of the essential Cdc13p protein to give rise to Cdc13-independent (or cap-independent) strains. Here we show that Cdc13-independent cells grow with persistently recognized DNA damage, which does not however result in a checkpoint activation; thus no defect in cell cycle progression is detectable. The absence of checkpoint signalling rather is due to the accumulation of mutations in checkpoint genes such as RAD24 or MEC1. Importantly, our results also show that cells that have lost the ability to adapt to persistent DNA damage, also are very much impaired in generating cap-independent cells. Altogether, these results show that while the capping process can be flexible, it takes a very specific genetic setup to allow a change from canonical capping to alternative capping. We hypothesize that in the alternative capping mode, genome integrity mechanisms are abrogated, which could cause increased mutation frequencies. These results from yeast have clear parallels in transformed human cancer cells and offer deeper insights into processes operating in pre-cancerous human cells that harbour eroded telomeres. Shared Science Publishers OG 2015-09-21 /pmc/articles/PMC5354583/ /pubmed/28357265 http://dx.doi.org/10.15698/mic2015.10.229 Text en https://creativecommons.org/licenses/by/4.0/ This is an open-access article released under the terms of the Creative Commons Attribution (CC BY) license, which allows the unrestricted use, distribution, and reproduction in any medium, provided the original author and source are acknowledged.
spellingShingle Microbiology
Mersaoui, Sofiane Y.
Gravel, Serge
Karpov, Victor
Wellinger, Raymund J.
DNA damage checkpoint adaptation genes are required for division of cells harbouring eroded telomeres
title DNA damage checkpoint adaptation genes are required for division of cells harbouring eroded telomeres
title_full DNA damage checkpoint adaptation genes are required for division of cells harbouring eroded telomeres
title_fullStr DNA damage checkpoint adaptation genes are required for division of cells harbouring eroded telomeres
title_full_unstemmed DNA damage checkpoint adaptation genes are required for division of cells harbouring eroded telomeres
title_short DNA damage checkpoint adaptation genes are required for division of cells harbouring eroded telomeres
title_sort dna damage checkpoint adaptation genes are required for division of cells harbouring eroded telomeres
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5354583/
https://www.ncbi.nlm.nih.gov/pubmed/28357265
http://dx.doi.org/10.15698/mic2015.10.229
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