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Improved poliovirus d-antigen yields by application of different Vero cell cultivation methods
Vero cells were grown adherent to microcarriers (Cytodex 1; 3 g L(−1)) using animal component free media in stirred-tank type bioreactors. Different strategies for media refreshment, daily media replacement (semi-batch), continuous media replacement (perfusion) and recirculation of media, were compa...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier Science
2014
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5355417/ https://www.ncbi.nlm.nih.gov/pubmed/24583004 http://dx.doi.org/10.1016/j.vaccine.2014.02.022 |