Efficient Precision Genome Editing in iPSCs via Genetic Co-targeting with Selection

Genome editing in induced pluripotent stem cells is currently hampered by the laborious and expensive nature of identifying homology-directed repair (HDR)-modified cells. We present an approach where isolation of cells bearing a selectable, HDR-mediated editing event at one locus enriches for HDR-me...

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Autores principales: Mitzelfelt, Katie A., McDermott-Roe, Chris, Grzybowski, Michael N., Marquez, Maribel, Kuo, Chieh-Ti, Riedel, Michael, Lai, Shuping, Choi, Melinda J., Kolander, Kurt D., Helbling, Daniel, Dimmock, David P., Battle, Michele A., Jou, Chuanchau J., Tristani-Firouzi, Martin, Verbsky, James W., Benjamin, Ivor J., Geurts, Aron M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5355643/
https://www.ncbi.nlm.nih.gov/pubmed/28238794
http://dx.doi.org/10.1016/j.stemcr.2017.01.021
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author Mitzelfelt, Katie A.
McDermott-Roe, Chris
Grzybowski, Michael N.
Marquez, Maribel
Kuo, Chieh-Ti
Riedel, Michael
Lai, Shuping
Choi, Melinda J.
Kolander, Kurt D.
Helbling, Daniel
Dimmock, David P.
Battle, Michele A.
Jou, Chuanchau J.
Tristani-Firouzi, Martin
Verbsky, James W.
Benjamin, Ivor J.
Geurts, Aron M.
author_facet Mitzelfelt, Katie A.
McDermott-Roe, Chris
Grzybowski, Michael N.
Marquez, Maribel
Kuo, Chieh-Ti
Riedel, Michael
Lai, Shuping
Choi, Melinda J.
Kolander, Kurt D.
Helbling, Daniel
Dimmock, David P.
Battle, Michele A.
Jou, Chuanchau J.
Tristani-Firouzi, Martin
Verbsky, James W.
Benjamin, Ivor J.
Geurts, Aron M.
author_sort Mitzelfelt, Katie A.
collection PubMed
description Genome editing in induced pluripotent stem cells is currently hampered by the laborious and expensive nature of identifying homology-directed repair (HDR)-modified cells. We present an approach where isolation of cells bearing a selectable, HDR-mediated editing event at one locus enriches for HDR-mediated edits at additional loci. This strategy, called co-targeting with selection, improves the probability of isolating cells bearing HDR-mediated variants and accelerates the production of disease models.
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spelling pubmed-53556432017-03-24 Efficient Precision Genome Editing in iPSCs via Genetic Co-targeting with Selection Mitzelfelt, Katie A. McDermott-Roe, Chris Grzybowski, Michael N. Marquez, Maribel Kuo, Chieh-Ti Riedel, Michael Lai, Shuping Choi, Melinda J. Kolander, Kurt D. Helbling, Daniel Dimmock, David P. Battle, Michele A. Jou, Chuanchau J. Tristani-Firouzi, Martin Verbsky, James W. Benjamin, Ivor J. Geurts, Aron M. Stem Cell Reports Report Genome editing in induced pluripotent stem cells is currently hampered by the laborious and expensive nature of identifying homology-directed repair (HDR)-modified cells. We present an approach where isolation of cells bearing a selectable, HDR-mediated editing event at one locus enriches for HDR-mediated edits at additional loci. This strategy, called co-targeting with selection, improves the probability of isolating cells bearing HDR-mediated variants and accelerates the production of disease models. Elsevier 2017-02-24 /pmc/articles/PMC5355643/ /pubmed/28238794 http://dx.doi.org/10.1016/j.stemcr.2017.01.021 Text en © 2017 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Report
Mitzelfelt, Katie A.
McDermott-Roe, Chris
Grzybowski, Michael N.
Marquez, Maribel
Kuo, Chieh-Ti
Riedel, Michael
Lai, Shuping
Choi, Melinda J.
Kolander, Kurt D.
Helbling, Daniel
Dimmock, David P.
Battle, Michele A.
Jou, Chuanchau J.
Tristani-Firouzi, Martin
Verbsky, James W.
Benjamin, Ivor J.
Geurts, Aron M.
Efficient Precision Genome Editing in iPSCs via Genetic Co-targeting with Selection
title Efficient Precision Genome Editing in iPSCs via Genetic Co-targeting with Selection
title_full Efficient Precision Genome Editing in iPSCs via Genetic Co-targeting with Selection
title_fullStr Efficient Precision Genome Editing in iPSCs via Genetic Co-targeting with Selection
title_full_unstemmed Efficient Precision Genome Editing in iPSCs via Genetic Co-targeting with Selection
title_short Efficient Precision Genome Editing in iPSCs via Genetic Co-targeting with Selection
title_sort efficient precision genome editing in ipscs via genetic co-targeting with selection
topic Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5355643/
https://www.ncbi.nlm.nih.gov/pubmed/28238794
http://dx.doi.org/10.1016/j.stemcr.2017.01.021
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