Molecular basis for functional switching of GFP by two disparate non-native post-translational modifications of a phenyl azide reaction handle

Through the genetic incorporation of a single phenyl azide group into superfolder GFP (sfGFP) at residue 148 we provide a molecular description of how this highly versatile chemical handle can be used to positively switch protein function in vitro and in vivo via either photochemistry or bioconjugat...

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Detalles Bibliográficos
Autores principales: Hartley, Andrew M., Worthy, Harley L., Reddington, Samuel C., Rizkallah, Pierre J., Jones, D. Dafydd
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2016
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5355941/
https://www.ncbi.nlm.nih.gov/pubmed/28451106
http://dx.doi.org/10.1039/c6sc00944a

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5355941/
https://www.ncbi.nlm.nih.gov/pubmed/28451106
http://dx.doi.org/10.1039/c6sc00944a

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