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Molecular basis for functional switching of GFP by two disparate non-native post-translational modifications of a phenyl azide reaction handle
Through the genetic incorporation of a single phenyl azide group into superfolder GFP (sfGFP) at residue 148 we provide a molecular description of how this highly versatile chemical handle can be used to positively switch protein function in vitro and in vivo via either photochemistry or bioconjugat...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Royal Society of Chemistry
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5355941/ https://www.ncbi.nlm.nih.gov/pubmed/28451106 http://dx.doi.org/10.1039/c6sc00944a |