A restriction-free method for gene reconstitution using two single-primer PCRs in parallel to generate compatible cohesive ends

BACKGROUND: Restriction-free (RF) cloning, a PCR-based method for the creation of custom DNA plasmids, allows for the insertion of any sequence into any plasmid vector at any desired position, independent of restriction sites and/or ligation. Here, we describe a simple and fast method for performing...

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Detalles Bibliográficos
Autores principales: Zeng, Fanli, Hao, Zhimin, Li, Pan, Meng, Yanan, Dong, Jingao, Lin, Yibin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5356277/
https://www.ncbi.nlm.nih.gov/pubmed/28302113
http://dx.doi.org/10.1186/s12896-017-0346-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5356277/
https://www.ncbi.nlm.nih.gov/pubmed/28302113
http://dx.doi.org/10.1186/s12896-017-0346-5

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