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A restriction-free method for gene reconstitution using two single-primer PCRs in parallel to generate compatible cohesive ends
BACKGROUND: Restriction-free (RF) cloning, a PCR-based method for the creation of custom DNA plasmids, allows for the insertion of any sequence into any plasmid vector at any desired position, independent of restriction sites and/or ligation. Here, we describe a simple and fast method for performing...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2017
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5356277/ https://www.ncbi.nlm.nih.gov/pubmed/28302113 http://dx.doi.org/10.1186/s12896-017-0346-5 |