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Implications of evolutionary engineering for growth and recombinant protein production in methanol-based growth media in the yeast Pichia pastoris
BACKGROUND: Pichia pastoris is a widely used eukaryotic expression host for recombinant protein production. Adaptive laboratory evolution (ALE) has been applied in a wide range of studies in order to improve strains for biotechnological purposes. In this context, the impact of long-term carbon sourc...
Autores principales: | Moser, Josef W., Prielhofer, Roland, Gerner, Samuel M., Graf, Alexandra B., Wilson, Iain B. H., Mattanovich, Diethard, Dragosits, Martin |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5356285/ https://www.ncbi.nlm.nih.gov/pubmed/28302114 http://dx.doi.org/10.1186/s12934-017-0661-5 |
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