How are base excision DNA repair pathways deployed in vivo?

Since the discovery of the base excision repair (BER) system for DNA more than 40 years ago, new branches of the pathway have been revealed at the biochemical level by in vitro studies. Largely for technical reasons, however, the confirmation of these subpathways in vivo has been elusive. We review...

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Detalles Bibliográficos
Autores principales: Thapar, Upasna, Demple, Bruce
Formato: Online Artículo Texto
Lenguaje:English
Publicado: F1000Research 2017
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5357041/
https://www.ncbi.nlm.nih.gov/pubmed/28357058
http://dx.doi.org/10.12688/f1000research.10538.1
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author Thapar, Upasna
Demple, Bruce
author_facet Thapar, Upasna
Demple, Bruce
author_sort Thapar, Upasna
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description Since the discovery of the base excision repair (BER) system for DNA more than 40 years ago, new branches of the pathway have been revealed at the biochemical level by in vitro studies. Largely for technical reasons, however, the confirmation of these subpathways in vivo has been elusive. We review methods that have been used to explore BER in mammalian cells, indicate where there are important knowledge gaps to fill, and suggest a way to address them.
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spelling pubmed-53570412017-03-28 How are base excision DNA repair pathways deployed in vivo? Thapar, Upasna Demple, Bruce F1000Res Review Since the discovery of the base excision repair (BER) system for DNA more than 40 years ago, new branches of the pathway have been revealed at the biochemical level by in vitro studies. Largely for technical reasons, however, the confirmation of these subpathways in vivo has been elusive. We review methods that have been used to explore BER in mammalian cells, indicate where there are important knowledge gaps to fill, and suggest a way to address them. F1000Research 2017-03-16 /pmc/articles/PMC5357041/ /pubmed/28357058 http://dx.doi.org/10.12688/f1000research.10538.1 Text en Copyright: © 2017 Thapar U and Demple B http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review
Thapar, Upasna
Demple, Bruce
How are base excision DNA repair pathways deployed in vivo?
title How are base excision DNA repair pathways deployed in vivo?
title_full How are base excision DNA repair pathways deployed in vivo?
title_fullStr How are base excision DNA repair pathways deployed in vivo?
title_full_unstemmed How are base excision DNA repair pathways deployed in vivo?
title_short How are base excision DNA repair pathways deployed in vivo?
title_sort how are base excision dna repair pathways deployed in vivo?
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5357041/
https://www.ncbi.nlm.nih.gov/pubmed/28357058
http://dx.doi.org/10.12688/f1000research.10538.1
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