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Direct Evidence of Lack of Colocalisation of Fluorescently Labelled Gold Labels Used in Correlative Light Electron Microscopy

Fluorescently labelled nanoparticles are routinely used in Correlative Light Electron Microscopy (CLEM) to combine the capabilities of two separate microscope platforms: fluorescent light microscopy (LM) and electron microscopy (EM). The inherent assumption is that the fluorescent label observed und...

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Detalles Bibliográficos
Autores principales: Miles, Benjamin T., Greenwood, Alexander B., Benito-Alifonso, David, Tanner, Hugh, Galan, M. Carmen, Verkade, Paul, Gersen, Henkjan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5357795/
https://www.ncbi.nlm.nih.gov/pubmed/28317888
http://dx.doi.org/10.1038/srep44666