Cbx3 maintains lineage specificity during neural differentiation

Chromobox homolog 3 (Cbx3/heterochromatin protein 1γ [HP1γ]) stimulates cell differentiation, but its mechanism is unknown. We found that Cbx3 binds to gene promoters upon differentiation of murine embryonic stem cells (ESCs) to neural progenitor cells (NPCs) and recruits the Mediator subunit Med26....

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Autores principales: Huang, Chengyang, Su, Trent, Xue, Yong, Cheng, Chen, Lay, Fides D., McKee, Robin A., Li, Meiyang, Vashisht, Ajay, Wohlschlegel, James, Novitch, Bennett G., Plath, Kathrin, Kurdistani, Siavash K., Carey, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2017
Materias:
Research Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5358721/
https://www.ncbi.nlm.nih.gov/pubmed/28270516
http://dx.doi.org/10.1101/gad.292169.116
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author Huang, Chengyang
Su, Trent
Xue, Yong
Cheng, Chen
Lay, Fides D.
McKee, Robin A.
Li, Meiyang
Vashisht, Ajay
Wohlschlegel, James
Novitch, Bennett G.
Plath, Kathrin
Kurdistani, Siavash K.
Carey, Michael
author_facet Huang, Chengyang
Su, Trent
Xue, Yong
Cheng, Chen
Lay, Fides D.
McKee, Robin A.
Li, Meiyang
Vashisht, Ajay
Wohlschlegel, James
Novitch, Bennett G.
Plath, Kathrin
Kurdistani, Siavash K.
Carey, Michael
author_sort Huang, Chengyang
collection PubMed
description Chromobox homolog 3 (Cbx3/heterochromatin protein 1γ [HP1γ]) stimulates cell differentiation, but its mechanism is unknown. We found that Cbx3 binds to gene promoters upon differentiation of murine embryonic stem cells (ESCs) to neural progenitor cells (NPCs) and recruits the Mediator subunit Med26. RNAi knockdown of either Cbx3 or Med26 inhibits neural differentiation while up-regulating genes involved in mesodermal lineage decisions. Thus, Cbx3 and Med26 together ensure the fidelity of lineage specification by enhancing the expression of neural genes and down-regulating genes specific to alternative fates.
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spelling pubmed-53587212017-08-01 Cbx3 maintains lineage specificity during neural differentiation Huang, Chengyang Su, Trent Xue, Yong Cheng, Chen Lay, Fides D. McKee, Robin A. Li, Meiyang Vashisht, Ajay Wohlschlegel, James Novitch, Bennett G. Plath, Kathrin Kurdistani, Siavash K. Carey, Michael Genes Dev Research Communication Chromobox homolog 3 (Cbx3/heterochromatin protein 1γ [HP1γ]) stimulates cell differentiation, but its mechanism is unknown. We found that Cbx3 binds to gene promoters upon differentiation of murine embryonic stem cells (ESCs) to neural progenitor cells (NPCs) and recruits the Mediator subunit Med26. RNAi knockdown of either Cbx3 or Med26 inhibits neural differentiation while up-regulating genes involved in mesodermal lineage decisions. Thus, Cbx3 and Med26 together ensure the fidelity of lineage specification by enhancing the expression of neural genes and down-regulating genes specific to alternative fates. Cold Spring Harbor Laboratory Press 2017-02-01 /pmc/articles/PMC5358721/ /pubmed/28270516 http://dx.doi.org/10.1101/gad.292169.116 Text en © 2017 Huang et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Research Communication
Huang, Chengyang
Su, Trent
Xue, Yong
Cheng, Chen
Lay, Fides D.
McKee, Robin A.
Li, Meiyang
Vashisht, Ajay
Wohlschlegel, James
Novitch, Bennett G.
Plath, Kathrin
Kurdistani, Siavash K.
Carey, Michael
Cbx3 maintains lineage specificity during neural differentiation
title Cbx3 maintains lineage specificity during neural differentiation
title_full Cbx3 maintains lineage specificity during neural differentiation
title_fullStr Cbx3 maintains lineage specificity during neural differentiation
title_full_unstemmed Cbx3 maintains lineage specificity during neural differentiation
title_short Cbx3 maintains lineage specificity during neural differentiation
title_sort cbx3 maintains lineage specificity during neural differentiation
topic Research Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5358721/
https://www.ncbi.nlm.nih.gov/pubmed/28270516
http://dx.doi.org/10.1101/gad.292169.116
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