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TDRD6 mediates early steps of spliceosome maturation in primary spermatocytes
Tudor containing protein 6 (TDRD6) is a male germ line-specific protein essential for chromatoid body (ChB) structure, elongated spermatid development and male fertility. Here we show that in meiotic prophase I spermatocytes TDRD6 interacts with the key protein arginine methyl transferase PRMT5, whi...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5358835/ https://www.ncbi.nlm.nih.gov/pubmed/28263986 http://dx.doi.org/10.1371/journal.pgen.1006660 |
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author | Akpınar, Müge Lesche, Mathias Fanourgakis, Grigorios Fu, Jun Anasstasiadis, Konstantinos Dahl, Andreas Jessberger, Rolf |
author_facet | Akpınar, Müge Lesche, Mathias Fanourgakis, Grigorios Fu, Jun Anasstasiadis, Konstantinos Dahl, Andreas Jessberger, Rolf |
author_sort | Akpınar, Müge |
collection | PubMed |
description | Tudor containing protein 6 (TDRD6) is a male germ line-specific protein essential for chromatoid body (ChB) structure, elongated spermatid development and male fertility. Here we show that in meiotic prophase I spermatocytes TDRD6 interacts with the key protein arginine methyl transferase PRMT5, which supports splicing. TDRD6 also associates with spliceosomal core protein SmB in the absence of RNA and in an arginine methylation dependent manner. In Tdrd6(-/-) diplotene spermatocytes PRMT5 association with SmB and arginine dimethylation of SmB are much reduced. TDRD6 deficiency impairs the assembly of spliceosomes, which feature 3.5-fold increased levels of U5 snRNPs. In the nucleus, these deficiencies in spliceosome maturation correlate with decreased numbers of SMN-positive bodies and Cajal bodies involved in nuclear snRNP maturation. Transcriptome analysis of TDRD6-deficient diplotene spermatocytes revealed high numbers of splicing defects such as aberrant usage of intron and exons as well as aberrant representation of splice junctions. Together, this study demonstrates a novel function of TDRD6 in spliceosome maturation and mRNA splicing in prophase I spermatocytes. |
format | Online Article Text |
id | pubmed-5358835 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-53588352017-04-06 TDRD6 mediates early steps of spliceosome maturation in primary spermatocytes Akpınar, Müge Lesche, Mathias Fanourgakis, Grigorios Fu, Jun Anasstasiadis, Konstantinos Dahl, Andreas Jessberger, Rolf PLoS Genet Research Article Tudor containing protein 6 (TDRD6) is a male germ line-specific protein essential for chromatoid body (ChB) structure, elongated spermatid development and male fertility. Here we show that in meiotic prophase I spermatocytes TDRD6 interacts with the key protein arginine methyl transferase PRMT5, which supports splicing. TDRD6 also associates with spliceosomal core protein SmB in the absence of RNA and in an arginine methylation dependent manner. In Tdrd6(-/-) diplotene spermatocytes PRMT5 association with SmB and arginine dimethylation of SmB are much reduced. TDRD6 deficiency impairs the assembly of spliceosomes, which feature 3.5-fold increased levels of U5 snRNPs. In the nucleus, these deficiencies in spliceosome maturation correlate with decreased numbers of SMN-positive bodies and Cajal bodies involved in nuclear snRNP maturation. Transcriptome analysis of TDRD6-deficient diplotene spermatocytes revealed high numbers of splicing defects such as aberrant usage of intron and exons as well as aberrant representation of splice junctions. Together, this study demonstrates a novel function of TDRD6 in spliceosome maturation and mRNA splicing in prophase I spermatocytes. Public Library of Science 2017-03-06 /pmc/articles/PMC5358835/ /pubmed/28263986 http://dx.doi.org/10.1371/journal.pgen.1006660 Text en © 2017 Akpınar et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Akpınar, Müge Lesche, Mathias Fanourgakis, Grigorios Fu, Jun Anasstasiadis, Konstantinos Dahl, Andreas Jessberger, Rolf TDRD6 mediates early steps of spliceosome maturation in primary spermatocytes |
title | TDRD6 mediates early steps of spliceosome maturation in primary spermatocytes |
title_full | TDRD6 mediates early steps of spliceosome maturation in primary spermatocytes |
title_fullStr | TDRD6 mediates early steps of spliceosome maturation in primary spermatocytes |
title_full_unstemmed | TDRD6 mediates early steps of spliceosome maturation in primary spermatocytes |
title_short | TDRD6 mediates early steps of spliceosome maturation in primary spermatocytes |
title_sort | tdrd6 mediates early steps of spliceosome maturation in primary spermatocytes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5358835/ https://www.ncbi.nlm.nih.gov/pubmed/28263986 http://dx.doi.org/10.1371/journal.pgen.1006660 |
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