A metal-mediated base pair that discriminates between the canonical pyrimidine nucleobases

A nucleoside analogue comprising the ligand 1H-imidazo[4,5-f][1,10]phenanthroline (P) was applied to develop a molecular beacon capable of discriminating the canonical nucleobases cytosine and thymine. The beacon is based on the formation of a stable Ag(+)-mediated base pair between P and cytosine,...

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Detalles Bibliográficos
Autores principales: Jash, Biswarup, Scharf, Philipp, Sandmann, Nikolas, Fonseca Guerra, Célia, Megger, Dominik A., Müller, Jens
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2017
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5360170/
https://www.ncbi.nlm.nih.gov/pubmed/28451274
http://dx.doi.org/10.1039/c6sc03482a
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author Jash, Biswarup
Scharf, Philipp
Sandmann, Nikolas
Fonseca Guerra, Célia
Megger, Dominik A.
Müller, Jens
author_facet Jash, Biswarup
Scharf, Philipp
Sandmann, Nikolas
Fonseca Guerra, Célia
Megger, Dominik A.
Müller, Jens
author_sort Jash, Biswarup
collection PubMed
description A nucleoside analogue comprising the ligand 1H-imidazo[4,5-f][1,10]phenanthroline (P) was applied to develop a molecular beacon capable of discriminating the canonical nucleobases cytosine and thymine. The beacon is based on the formation of a stable Ag(+)-mediated base pair between P and cytosine, whereas the presence of Ag(+) strongly destabilizes nucleic acids comprising an artificial base pair between P and thymine. Metal-mediated base pair formation was investigated by temperature-dependent UV spectroscopy and CD spectroscopy and complemented by extensive DFT calculations. The molecular beacon significantly extends the application spectrum of nucleic acids with metal-mediated base pairs. It is of potential use in the detection of single-nucleotide polymorphisms.
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spelling pubmed-53601702017-04-27 A metal-mediated base pair that discriminates between the canonical pyrimidine nucleobases Jash, Biswarup Scharf, Philipp Sandmann, Nikolas Fonseca Guerra, Célia Megger, Dominik A. Müller, Jens Chem Sci Chemistry A nucleoside analogue comprising the ligand 1H-imidazo[4,5-f][1,10]phenanthroline (P) was applied to develop a molecular beacon capable of discriminating the canonical nucleobases cytosine and thymine. The beacon is based on the formation of a stable Ag(+)-mediated base pair between P and cytosine, whereas the presence of Ag(+) strongly destabilizes nucleic acids comprising an artificial base pair between P and thymine. Metal-mediated base pair formation was investigated by temperature-dependent UV spectroscopy and CD spectroscopy and complemented by extensive DFT calculations. The molecular beacon significantly extends the application spectrum of nucleic acids with metal-mediated base pairs. It is of potential use in the detection of single-nucleotide polymorphisms. Royal Society of Chemistry 2017-02-01 2016-10-12 /pmc/articles/PMC5360170/ /pubmed/28451274 http://dx.doi.org/10.1039/c6sc03482a Text en This journal is © The Royal Society of Chemistry 2016 http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 Unported License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Chemistry
Jash, Biswarup
Scharf, Philipp
Sandmann, Nikolas
Fonseca Guerra, Célia
Megger, Dominik A.
Müller, Jens
A metal-mediated base pair that discriminates between the canonical pyrimidine nucleobases
title A metal-mediated base pair that discriminates between the canonical pyrimidine nucleobases
title_full A metal-mediated base pair that discriminates between the canonical pyrimidine nucleobases
title_fullStr A metal-mediated base pair that discriminates between the canonical pyrimidine nucleobases
title_full_unstemmed A metal-mediated base pair that discriminates between the canonical pyrimidine nucleobases
title_short A metal-mediated base pair that discriminates between the canonical pyrimidine nucleobases
title_sort metal-mediated base pair that discriminates between the canonical pyrimidine nucleobases
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5360170/
https://www.ncbi.nlm.nih.gov/pubmed/28451274
http://dx.doi.org/10.1039/c6sc03482a
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