Cargando…
Diacerein inhibits the pro-atherogenic & pro-inflammatory effects of IL-1 on human keratinocytes & endothelial cells
We investigated IL-1-induced regulation of genes related to inflammation and atherogenesis in human keratinocytes and endothelial cells, and if ‘diacerein’, an oral IL-1 inhibiting drug currently approved for use in osteoarthritis, would reverse IL-1’s effects on these cells. Primary human keratinoc...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5360272/ https://www.ncbi.nlm.nih.gov/pubmed/28323859 http://dx.doi.org/10.1371/journal.pone.0173981 |
_version_ | 1782516561640161280 |
---|---|
author | Mohan, Girish C. Zhang, Huayi Bao, Lei Many, Benjamin Chan, Lawrence S. |
author_facet | Mohan, Girish C. Zhang, Huayi Bao, Lei Many, Benjamin Chan, Lawrence S. |
author_sort | Mohan, Girish C. |
collection | PubMed |
description | We investigated IL-1-induced regulation of genes related to inflammation and atherogenesis in human keratinocytes and endothelial cells, and if ‘diacerein’, an oral IL-1 inhibiting drug currently approved for use in osteoarthritis, would reverse IL-1’s effects on these cells. Primary human keratinocytes and coronary artery endothelial cells were treated with either IL-1α or IL-1β, with and without diacerein. Using PCR-array, we assessed differential gene-expression regulated by IL-1 and diacerein. We identified 34 pro-atherogenic genes in endothelial cells and 68 pro-inflammatory genes in keratinocytes significantly (p<0.05) regulated at least 2-fold by IL-1, in comparison to control. Diacerein completely or partially reversed this regulation on almost all genes. Using ELISA, we confirmed diacerein’s ability to reverse IL-1-driven gene-regulation of 11 selected factors, at the protein level. The results support a novel idea that diacerein acts as an inhibitor of the pro-atherogenic and pro-inflammatory effects of IL-1. Diacerein may have therapeutic applications to diminish IL-1-induced skin inflammation in psoriasis and attenuate IL-1-induced development of atherosclerosis. Further investigation into diacerein’s effect on skin inflammation, atherogenesis and cardiovascular risk in animal models or humans is warranted. |
format | Online Article Text |
id | pubmed-5360272 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-53602722017-04-06 Diacerein inhibits the pro-atherogenic & pro-inflammatory effects of IL-1 on human keratinocytes & endothelial cells Mohan, Girish C. Zhang, Huayi Bao, Lei Many, Benjamin Chan, Lawrence S. PLoS One Research Article We investigated IL-1-induced regulation of genes related to inflammation and atherogenesis in human keratinocytes and endothelial cells, and if ‘diacerein’, an oral IL-1 inhibiting drug currently approved for use in osteoarthritis, would reverse IL-1’s effects on these cells. Primary human keratinocytes and coronary artery endothelial cells were treated with either IL-1α or IL-1β, with and without diacerein. Using PCR-array, we assessed differential gene-expression regulated by IL-1 and diacerein. We identified 34 pro-atherogenic genes in endothelial cells and 68 pro-inflammatory genes in keratinocytes significantly (p<0.05) regulated at least 2-fold by IL-1, in comparison to control. Diacerein completely or partially reversed this regulation on almost all genes. Using ELISA, we confirmed diacerein’s ability to reverse IL-1-driven gene-regulation of 11 selected factors, at the protein level. The results support a novel idea that diacerein acts as an inhibitor of the pro-atherogenic and pro-inflammatory effects of IL-1. Diacerein may have therapeutic applications to diminish IL-1-induced skin inflammation in psoriasis and attenuate IL-1-induced development of atherosclerosis. Further investigation into diacerein’s effect on skin inflammation, atherogenesis and cardiovascular risk in animal models or humans is warranted. Public Library of Science 2017-03-21 /pmc/articles/PMC5360272/ /pubmed/28323859 http://dx.doi.org/10.1371/journal.pone.0173981 Text en © 2017 Mohan et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Mohan, Girish C. Zhang, Huayi Bao, Lei Many, Benjamin Chan, Lawrence S. Diacerein inhibits the pro-atherogenic & pro-inflammatory effects of IL-1 on human keratinocytes & endothelial cells |
title | Diacerein inhibits the pro-atherogenic & pro-inflammatory effects of IL-1 on human keratinocytes & endothelial cells |
title_full | Diacerein inhibits the pro-atherogenic & pro-inflammatory effects of IL-1 on human keratinocytes & endothelial cells |
title_fullStr | Diacerein inhibits the pro-atherogenic & pro-inflammatory effects of IL-1 on human keratinocytes & endothelial cells |
title_full_unstemmed | Diacerein inhibits the pro-atherogenic & pro-inflammatory effects of IL-1 on human keratinocytes & endothelial cells |
title_short | Diacerein inhibits the pro-atherogenic & pro-inflammatory effects of IL-1 on human keratinocytes & endothelial cells |
title_sort | diacerein inhibits the pro-atherogenic & pro-inflammatory effects of il-1 on human keratinocytes & endothelial cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5360272/ https://www.ncbi.nlm.nih.gov/pubmed/28323859 http://dx.doi.org/10.1371/journal.pone.0173981 |
work_keys_str_mv | AT mohangirishc diacereininhibitstheproatherogenicproinflammatoryeffectsofil1onhumankeratinocytesendothelialcells AT zhanghuayi diacereininhibitstheproatherogenicproinflammatoryeffectsofil1onhumankeratinocytesendothelialcells AT baolei diacereininhibitstheproatherogenicproinflammatoryeffectsofil1onhumankeratinocytesendothelialcells AT manybenjamin diacereininhibitstheproatherogenicproinflammatoryeffectsofil1onhumankeratinocytesendothelialcells AT chanlawrences diacereininhibitstheproatherogenicproinflammatoryeffectsofil1onhumankeratinocytesendothelialcells |