The importance of arginine codons AGA and AGG for the expression in E. coli of triosephosphate isomerase from seven different species

Rare arginine codons AGA and AGG affect the heterologous expression of proteins in Eschericha coli. The tRNAs necessary for protein synthesis are scarce in E. coli strain BL21(DE3) pLysS and plentiful in strain BL21(DE3) CodonPlus −RIL. We evaluated in both bacterial strains the effect of these rare...

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Detalles Bibliográficos
Autores principales: Aguirre-López, Beatriz, Cabrera, Nallely, de Gómez-Puyou, Marietta Tuena, Perez-Montfort, Ruy, Gómez-Puyou, Armando
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5361128/
https://www.ncbi.nlm.nih.gov/pubmed/28352562
http://dx.doi.org/10.1016/j.btre.2017.01.002

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5361128/
https://www.ncbi.nlm.nih.gov/pubmed/28352562
http://dx.doi.org/10.1016/j.btre.2017.01.002

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