Evaluation of Different Oligonucleotide Base Substitutions at CpG Binding sites in Multiplex Bisulfite-PCR sequencing

Multiplex bisulfite-PCR sequencing is a convenient and scalable method for the quantitative determination of the methylation state of target DNA regions. A challenge of this application is the presence of CpGs in the same region where primers are being placed. A common solution to the presence of Cp...

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Detalles Bibliográficos
Autores principales: Lu, Jennifer, Ru, Kelin, Candiloro, Ida, Dobrovic, Alexander, Korbie, Darren, Trau, Matt
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5361148/
https://www.ncbi.nlm.nih.gov/pubmed/28327639
http://dx.doi.org/10.1038/srep45096

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5361148/
https://www.ncbi.nlm.nih.gov/pubmed/28327639
http://dx.doi.org/10.1038/srep45096

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