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Evaluation of Different Oligonucleotide Base Substitutions at CpG Binding sites in Multiplex Bisulfite-PCR sequencing
Multiplex bisulfite-PCR sequencing is a convenient and scalable method for the quantitative determination of the methylation state of target DNA regions. A challenge of this application is the presence of CpGs in the same region where primers are being placed. A common solution to the presence of Cp...
Autores principales: | Lu, Jennifer, Ru, Kelin, Candiloro, Ida, Dobrovic, Alexander, Korbie, Darren, Trau, Matt |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5361148/ https://www.ncbi.nlm.nih.gov/pubmed/28327639 http://dx.doi.org/10.1038/srep45096 |
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