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Analysis of Anti-Influenza Virus Neuraminidase Antibodies in Children, Adults, and the Elderly by ELISA and Enzyme Inhibition: Evidence for Original Antigenic Sin

Antibody responses to influenza virus hemagglutinin provide protection against infection and are well studied. Less is known about the human antibody responses to the second surface glycoprotein, neuraminidase. Here, we assessed human antibody reactivity to a panel of N1, N2, and influenza B virus n...

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Autores principales: Rajendran, Madhusudan, Nachbagauer, Raffael, Ermler, Megan E., Bunduc, Paul, Amanat, Fatima, Izikson, Ruvim, Cox, Manon, Palese, Peter, Eichelberger, Maryna, Krammer, Florian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5362038/
https://www.ncbi.nlm.nih.gov/pubmed/28325769
http://dx.doi.org/10.1128/mBio.02281-16
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author Rajendran, Madhusudan
Nachbagauer, Raffael
Ermler, Megan E.
Bunduc, Paul
Amanat, Fatima
Izikson, Ruvim
Cox, Manon
Palese, Peter
Eichelberger, Maryna
Krammer, Florian
author_facet Rajendran, Madhusudan
Nachbagauer, Raffael
Ermler, Megan E.
Bunduc, Paul
Amanat, Fatima
Izikson, Ruvim
Cox, Manon
Palese, Peter
Eichelberger, Maryna
Krammer, Florian
author_sort Rajendran, Madhusudan
collection PubMed
description Antibody responses to influenza virus hemagglutinin provide protection against infection and are well studied. Less is known about the human antibody responses to the second surface glycoprotein, neuraminidase. Here, we assessed human antibody reactivity to a panel of N1, N2, and influenza B virus neuraminidases in different age groups, including children, adults, and the elderly. Using enzyme-linked immunosorbent assays (ELISA), we determined the breadth, magnitude, and isotype distribution of neuraminidase antibody responses to historic, current, and avian strains, as well as to recent isolates to which these individuals have not been exposed. It appears that antibody levels against N1 neuraminidases were lower than those against N2 or B neuraminidases. The anti-neuraminidase antibody levels increased with age and were, in general, highest against strains that circulated during the childhood of the tested individuals, providing evidence for “original antigenic sin.” Titers measured by ELISA correlated well with titers measured by the neuraminidase inhibition assays. However, in the case of the 2009 pandemic H1N1 virus, we found evidence of interference from antibodies binding to the conserved stalk domain of the hemagglutinin. In conclusion, we found that antibodies against the neuraminidase differ in magnitude and breadth between subtypes and age groups in the human population. (This study has been registered at ClinicalTrials.gov under registration no. NCT00336453, NCT00539981, and NCT00395174.)
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spelling pubmed-53620382017-03-24 Analysis of Anti-Influenza Virus Neuraminidase Antibodies in Children, Adults, and the Elderly by ELISA and Enzyme Inhibition: Evidence for Original Antigenic Sin Rajendran, Madhusudan Nachbagauer, Raffael Ermler, Megan E. Bunduc, Paul Amanat, Fatima Izikson, Ruvim Cox, Manon Palese, Peter Eichelberger, Maryna Krammer, Florian mBio Research Article Antibody responses to influenza virus hemagglutinin provide protection against infection and are well studied. Less is known about the human antibody responses to the second surface glycoprotein, neuraminidase. Here, we assessed human antibody reactivity to a panel of N1, N2, and influenza B virus neuraminidases in different age groups, including children, adults, and the elderly. Using enzyme-linked immunosorbent assays (ELISA), we determined the breadth, magnitude, and isotype distribution of neuraminidase antibody responses to historic, current, and avian strains, as well as to recent isolates to which these individuals have not been exposed. It appears that antibody levels against N1 neuraminidases were lower than those against N2 or B neuraminidases. The anti-neuraminidase antibody levels increased with age and were, in general, highest against strains that circulated during the childhood of the tested individuals, providing evidence for “original antigenic sin.” Titers measured by ELISA correlated well with titers measured by the neuraminidase inhibition assays. However, in the case of the 2009 pandemic H1N1 virus, we found evidence of interference from antibodies binding to the conserved stalk domain of the hemagglutinin. In conclusion, we found that antibodies against the neuraminidase differ in magnitude and breadth between subtypes and age groups in the human population. (This study has been registered at ClinicalTrials.gov under registration no. NCT00336453, NCT00539981, and NCT00395174.) American Society for Microbiology 2017-03-21 /pmc/articles/PMC5362038/ /pubmed/28325769 http://dx.doi.org/10.1128/mBio.02281-16 Text en Copyright © 2017 Rajendran et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Rajendran, Madhusudan
Nachbagauer, Raffael
Ermler, Megan E.
Bunduc, Paul
Amanat, Fatima
Izikson, Ruvim
Cox, Manon
Palese, Peter
Eichelberger, Maryna
Krammer, Florian
Analysis of Anti-Influenza Virus Neuraminidase Antibodies in Children, Adults, and the Elderly by ELISA and Enzyme Inhibition: Evidence for Original Antigenic Sin
title Analysis of Anti-Influenza Virus Neuraminidase Antibodies in Children, Adults, and the Elderly by ELISA and Enzyme Inhibition: Evidence for Original Antigenic Sin
title_full Analysis of Anti-Influenza Virus Neuraminidase Antibodies in Children, Adults, and the Elderly by ELISA and Enzyme Inhibition: Evidence for Original Antigenic Sin
title_fullStr Analysis of Anti-Influenza Virus Neuraminidase Antibodies in Children, Adults, and the Elderly by ELISA and Enzyme Inhibition: Evidence for Original Antigenic Sin
title_full_unstemmed Analysis of Anti-Influenza Virus Neuraminidase Antibodies in Children, Adults, and the Elderly by ELISA and Enzyme Inhibition: Evidence for Original Antigenic Sin
title_short Analysis of Anti-Influenza Virus Neuraminidase Antibodies in Children, Adults, and the Elderly by ELISA and Enzyme Inhibition: Evidence for Original Antigenic Sin
title_sort analysis of anti-influenza virus neuraminidase antibodies in children, adults, and the elderly by elisa and enzyme inhibition: evidence for original antigenic sin
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5362038/
https://www.ncbi.nlm.nih.gov/pubmed/28325769
http://dx.doi.org/10.1128/mBio.02281-16
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