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The Cation−π Interaction Enables a Halo-Tag Fluorogenic Probe for Fast No-Wash Live Cell Imaging and Gel-Free Protein Quantification

[Image: see text] The design of fluorogenic probes for a Halo tag is highly desirable but challenging. Previous work achieved this goal by controlling the chemical switch of spirolactones upon the covalent conjugation between the Halo tag and probes or by incorporating a “channel dye” into the subst...

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Detalles Bibliográficos
Autores principales: Liu, Yu, Miao, Kun, Dunham, Noah P., Liu, Hongbin, Fares, Matthew, Boal, Amie K., Li, Xiaosong, Zhang, Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2017
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5362743/
https://www.ncbi.nlm.nih.gov/pubmed/28221782
http://dx.doi.org/10.1021/acs.biochem.7b00056