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Patient-specific three-dimensional explant spheroids derived from human nasal airway epithelium: a simple methodological approach for ex vivo studies of primary ciliary dyskinesia

BACKGROUND: Three-dimensional explant spheroid formation is an ex vivo technique previously used in studies of airway epithelial ion and water transport. Explanted cells and sheets of nasal epithelium form fully differentiated spheroids enclosing a partly fluid-filled lumen with the ciliated apical...

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Detalles Bibliográficos
Autores principales: Marthin, June Kehlet, Stevens, Elizabeth Munkebjerg, Larsen, Lars Allan, Christensen, Søren Tvorup, Nielsen, Kim Gjerum
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5364668/
https://www.ncbi.nlm.nih.gov/pubmed/28344781
http://dx.doi.org/10.1186/s13630-017-0049-5