Expression, Purification, and Monitoring of Conformational Changes of hCB2 TMH67H8 in Different Membrane-Mimetic Lipid Mixtures Using Circular Dichroism and NMR Techniques

This work was intended to develop self-assembly lipids for incorporating G-protein coupled receptors (GPCRs) in order to improve the success rate for nuclear magnetic resonance spectroscopy (NMR) structural elucidation. We hereby report the expression and purification of uniformly (15)N-labeled huma...

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Detalles Bibliográficos
Autores principales: Tiburu, Elvis K., Zhuang, Jianqin, Fleischer, Heidimarie N. A., Arthur, Patrick K., Awandare, Gordon A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5371971/
https://www.ncbi.nlm.nih.gov/pubmed/28218648
http://dx.doi.org/10.3390/membranes7010010

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5371971/
https://www.ncbi.nlm.nih.gov/pubmed/28218648
http://dx.doi.org/10.3390/membranes7010010

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