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Partial Immunoblotting of 2D-Gels: A Novel Method to Identify Post-Translationally Modified Proteins Exemplified for the Myelin Acetylome

Post-translational modifications (PTMs) play a key role in regulating protein function, yet their identification is technically demanding. Here, we present a straightforward workflow to systematically identify post-translationally modified proteins based on two-dimensional gel electrophoresis. Upon...

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Detalles Bibliográficos
Autores principales: Kusch, Kathrin, Uecker, Marina, Liepold, Thomas, Möbius, Wiebke, Hoffmann, Christian, Neumann, Heinz, Werner, Hauke B., Jahn, Olaf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5372224/
https://www.ncbi.nlm.nih.gov/pubmed/28248254
http://dx.doi.org/10.3390/proteomes5010003