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Evaluation and Adaptation of a Laboratory-Based cDNA Library Preparation Protocol for Retrospective Sequencing of Archived MicroRNAs from up to 35-Year-Old Clinical FFPE Specimens

Formalin-fixed paraffin-embedded (FFPE) specimens, when used in conjunction with patient clinical data history, represent an invaluable resource for molecular studies of cancer. Even though nucleic acids extracted from archived FFPE tissues are degraded, their molecular analysis has become possible....

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Detalles Bibliográficos
Autores principales: Loudig, Olivier, Wang, Tao, Ye, Kenny, Lin, Juan, Wang, Yihong, Ramnauth, Andrew, Liu, Christina, Stark, Azadeh, Chitale, Dhananjay, Greenlee, Robert, Multerer, Deborah, Honda, Stacey, Daida, Yihe, Spencer Feigelson, Heather, Glass, Andrew, Couch, Fergus J., Rohan, Thomas, Ben-Dov, Iddo Z.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5372640/
https://www.ncbi.nlm.nih.gov/pubmed/28335433
http://dx.doi.org/10.3390/ijms18030627