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Next-Generation Sequencing-Based RiboMethSeq Protocol for Analysis of tRNA 2′-O-Methylation

Analysis of RNA modifications by traditional physico-chemical approaches is labor intensive, requires substantial amounts of input material and only allows site-by-site measurements. The recent development of qualitative and quantitative approaches based on next-generation sequencing (NGS) opens new...

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Detalles Bibliográficos
Autores principales: Marchand, Virginie, Pichot, Florian, Thüring, Kathrin, Ayadi, Lilia, Freund, Isabel, Dalpke, Alexander, Helm, Mark, Motorin, Yuri
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5372725/
https://www.ncbi.nlm.nih.gov/pubmed/28208788
http://dx.doi.org/10.3390/biom7010013