Short Communication: Potential Risk of Replication-Competent Virus in HIV-1 Env-Pseudotyped Virus Preparations

Env-pseudotyped viruses are valuable reagents for studies of HIV-1 neutralizing antibodies. It is often assumed that all pseudovirus particles are capable of only a single round of infection, making them a safe alternative to work with live HIV-1. In this study, we show that some Env-pseudotyped vir...

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Autores principales: Bilska, Miroslawa, Tang, Haili, Montefiori, David C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mary Ann Liebert, Inc. 2017
Materias:
Virology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5372769/
https://www.ncbi.nlm.nih.gov/pubmed/27758116
http://dx.doi.org/10.1089/aid.2016.0142
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author Bilska, Miroslawa
Tang, Haili
Montefiori, David C.
author_facet Bilska, Miroslawa
Tang, Haili
Montefiori, David C.
author_sort Bilska, Miroslawa
collection PubMed
description Env-pseudotyped viruses are valuable reagents for studies of HIV-1 neutralizing antibodies. It is often assumed that all pseudovirus particles are capable of only a single round of infection, making them a safe alternative to work with live HIV-1. In this study, we show that some Env-pseudotyped virus preparations give rise to low levels of replication-competent virus. These levels did not compromise results in the TZM-bl neutralization assay; however, their presence highlights a need to adhere to the same level of biosafety when working with Env-pseudotyped viruses that are required for work with replication competent HIV-1.
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spelling pubmed-53727692017-05-03 Short Communication: Potential Risk of Replication-Competent Virus in HIV-1 Env-Pseudotyped Virus Preparations Bilska, Miroslawa Tang, Haili Montefiori, David C. AIDS Res Hum Retroviruses Virology Env-pseudotyped viruses are valuable reagents for studies of HIV-1 neutralizing antibodies. It is often assumed that all pseudovirus particles are capable of only a single round of infection, making them a safe alternative to work with live HIV-1. In this study, we show that some Env-pseudotyped virus preparations give rise to low levels of replication-competent virus. These levels did not compromise results in the TZM-bl neutralization assay; however, their presence highlights a need to adhere to the same level of biosafety when working with Env-pseudotyped viruses that are required for work with replication competent HIV-1. Mary Ann Liebert, Inc. 2017-04-01 2017-04-01 /pmc/articles/PMC5372769/ /pubmed/27758116 http://dx.doi.org/10.1089/aid.2016.0142 Text en © Miroslawa Bilska, et al., 2017; Published by Mary Ann Liebert, Inc. This Open Access article is distributed under the terms of the Creative Commons Attribution Noncommercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Virology
Bilska, Miroslawa
Tang, Haili
Montefiori, David C.
Short Communication: Potential Risk of Replication-Competent Virus in HIV-1 Env-Pseudotyped Virus Preparations
title Short Communication: Potential Risk of Replication-Competent Virus in HIV-1 Env-Pseudotyped Virus Preparations
title_full Short Communication: Potential Risk of Replication-Competent Virus in HIV-1 Env-Pseudotyped Virus Preparations
title_fullStr Short Communication: Potential Risk of Replication-Competent Virus in HIV-1 Env-Pseudotyped Virus Preparations
title_full_unstemmed Short Communication: Potential Risk of Replication-Competent Virus in HIV-1 Env-Pseudotyped Virus Preparations
title_short Short Communication: Potential Risk of Replication-Competent Virus in HIV-1 Env-Pseudotyped Virus Preparations
title_sort short communication: potential risk of replication-competent virus in hiv-1 env-pseudotyped virus preparations
topic Virology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5372769/
https://www.ncbi.nlm.nih.gov/pubmed/27758116
http://dx.doi.org/10.1089/aid.2016.0142
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