Protein aggregation with poly(vinyl) alcohol surfactant reduces double emulsion-encapsulated mammalian cell-free expression

Development of artificial cell models requires encapsulation of biomolecules within membrane-bound compartments. There have been limited studies of using mammalian cell-free expression (CFE) system as the ‘cytosol’ of artificial cells. We exploit glass capillary droplet microfluidics for the encapsu...

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Detalles Bibliográficos
Autores principales: Ho, Kenneth K. Y., Lee, Jin Woo, Durand, Grégory, Majumder, Sagardip, Liu, Allen P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5373588/
https://www.ncbi.nlm.nih.gov/pubmed/28358875
http://dx.doi.org/10.1371/journal.pone.0174689

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5373588/
https://www.ncbi.nlm.nih.gov/pubmed/28358875
http://dx.doi.org/10.1371/journal.pone.0174689

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