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Looking for ugly ducklings: The role of the stability of BrdU-antibody complex and the improved method of the detection of DNA replication

5-Bromo-2′-deoxyuridine (BrdU) labelling and immunostaining is commonly used for the detection of DNA replication using specific antibodies. Previously, we found that these antibodies significantly differ in their affinity to BrdU. Our present data showed that one of the reasons for the differences...

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Detalles Bibliográficos
Autores principales: Ligasová, Anna, Konečný, Petr, Frydrych, Ivo, Koberna, Karel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5373633/
https://www.ncbi.nlm.nih.gov/pubmed/28358913
http://dx.doi.org/10.1371/journal.pone.0174893