Rational mutagenesis by engineering disulphide bonds improves Kluyveromyces lactis beta-galactosidase for high-temperature industrial applications

Kluyveromyces lactis β-galactosidase (Kl-β-Gal) is one of the most important enzymes in the dairy industry. The poor stability of this enzyme limits its use in the synthesis of galactooligosaccharides (GOS) and other applications requiring high operational temperature. To obtain thermoresistant vari...

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Detalles Bibliográficos
Autores principales: Rico-Díaz, Agustín, Álvarez-Cao, María-Efigenia, Escuder-Rodríguez, Juan-José, González-Siso, María-Isabel, Cerdán, M. Esperanza, Becerra, Manuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5374532/
https://www.ncbi.nlm.nih.gov/pubmed/28361909
http://dx.doi.org/10.1038/srep45535

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5374532/
https://www.ncbi.nlm.nih.gov/pubmed/28361909
http://dx.doi.org/10.1038/srep45535

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