Development of a genus-specific next generation sequencing approach for sensitive and quantitative determination of the Legionella microbiome in freshwater systems

BACKGROUND: Next Generation Sequencing (NGS) has revolutionized the analysis of natural and man-made microbial communities by using universal primers for bacteria in a PCR based approach targeting the 16S rRNA gene. In our study we narrowed primer specificity to a single, monophyletic genus because...

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Detalles Bibliográficos
Autores principales: Pereira, Rui P. A., Peplies, Jörg, Brettar, Ingrid, Höfle, Manfred G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5374610/
https://www.ncbi.nlm.nih.gov/pubmed/28359254
http://dx.doi.org/10.1186/s12866-017-0987-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5374610/
https://www.ncbi.nlm.nih.gov/pubmed/28359254
http://dx.doi.org/10.1186/s12866-017-0987-5

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