Enzyme-dependent fluorescence recovery of NADH after photobleaching to assess dehydrogenase activity of isolated perfused hearts

Reduction of NAD(+) by dehydrogenase enzymes to form NADH is a key component of cellular metabolism. In cellular preparations and isolated mitochondria suspensions, enzyme-dependent fluorescence recovery after photobleaching (ED-FRAP) of NADH has been shown to be an effective approach for measuring...

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Detalles Bibliográficos
Autores principales: Moreno, Angel, Kuzmiak-Glancy, Sarah, Jaimes, Rafael, Kay, Matthew W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5374639/
https://www.ncbi.nlm.nih.gov/pubmed/28361886
http://dx.doi.org/10.1038/srep45744

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5374639/
https://www.ncbi.nlm.nih.gov/pubmed/28361886
http://dx.doi.org/10.1038/srep45744

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