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Atomic resolution structures from fragmented protein crystals by the cryoEM method MicroED
Crystallographic analysis of macromolecules depends on large, well-ordered crystals, which often require significant effort to obtain. Even sizable crystals sometimes suffer from pathologies that render them inappropriate for high-resolution structure determination. Here we show that fragmentation o...
| Autores principales: | , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2017
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5376236/ https://www.ncbi.nlm.nih.gov/pubmed/28192420 http://dx.doi.org/10.1038/nmeth.4178 |
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| author | de la Cruz, M. Jason Hattne, Johan Shi, Dan Seidler, Paul Rodriguez, Jose Reyes, Francis E. Sawaya, Michael R. Cascio, Duilio Weiss, Simon C. Kim, Sun Kyung Hinck, Cynthia S. Hinck, Andrew P. Calero, Guillermo Eisenberg, David Gonen, Tamir |
| author_facet | de la Cruz, M. Jason Hattne, Johan Shi, Dan Seidler, Paul Rodriguez, Jose Reyes, Francis E. Sawaya, Michael R. Cascio, Duilio Weiss, Simon C. Kim, Sun Kyung Hinck, Cynthia S. Hinck, Andrew P. Calero, Guillermo Eisenberg, David Gonen, Tamir |
| author_sort | de la Cruz, M. Jason |
| collection | PubMed |
| description | Crystallographic analysis of macromolecules depends on large, well-ordered crystals, which often require significant effort to obtain. Even sizable crystals sometimes suffer from pathologies that render them inappropriate for high-resolution structure determination. Here we show that fragmentation of large, imperfect crystals can provide a simple path for high-resolution structure determination by serial femtosecond crystallography or the cryoEM method MicroED. |
| format | Online Article Text |
| id | pubmed-5376236 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2017 |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-53762362017-08-13 Atomic resolution structures from fragmented protein crystals by the cryoEM method MicroED de la Cruz, M. Jason Hattne, Johan Shi, Dan Seidler, Paul Rodriguez, Jose Reyes, Francis E. Sawaya, Michael R. Cascio, Duilio Weiss, Simon C. Kim, Sun Kyung Hinck, Cynthia S. Hinck, Andrew P. Calero, Guillermo Eisenberg, David Gonen, Tamir Nat Methods Article Crystallographic analysis of macromolecules depends on large, well-ordered crystals, which often require significant effort to obtain. Even sizable crystals sometimes suffer from pathologies that render them inappropriate for high-resolution structure determination. Here we show that fragmentation of large, imperfect crystals can provide a simple path for high-resolution structure determination by serial femtosecond crystallography or the cryoEM method MicroED. 2017-02-13 /pmc/articles/PMC5376236/ /pubmed/28192420 http://dx.doi.org/10.1038/nmeth.4178 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
| spellingShingle | Article de la Cruz, M. Jason Hattne, Johan Shi, Dan Seidler, Paul Rodriguez, Jose Reyes, Francis E. Sawaya, Michael R. Cascio, Duilio Weiss, Simon C. Kim, Sun Kyung Hinck, Cynthia S. Hinck, Andrew P. Calero, Guillermo Eisenberg, David Gonen, Tamir Atomic resolution structures from fragmented protein crystals by the cryoEM method MicroED |
| title | Atomic resolution structures from fragmented protein crystals by the cryoEM method MicroED |
| title_full | Atomic resolution structures from fragmented protein crystals by the cryoEM method MicroED |
| title_fullStr | Atomic resolution structures from fragmented protein crystals by the cryoEM method MicroED |
| title_full_unstemmed | Atomic resolution structures from fragmented protein crystals by the cryoEM method MicroED |
| title_short | Atomic resolution structures from fragmented protein crystals by the cryoEM method MicroED |
| title_sort | atomic resolution structures from fragmented protein crystals by the cryoem method microed |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5376236/ https://www.ncbi.nlm.nih.gov/pubmed/28192420 http://dx.doi.org/10.1038/nmeth.4178 |
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