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Analysis of the mechanism by which nerve growth factor promotes callus formation in mice with tibial fracture

The aim of the present study was to analyze the mechanism by which nerve growth factor (NGF) promotes callus formation in mice with tibial fracture. NGF transgenic homozygotic mice and NGF wild homozygotic mice were selected to construct non-stabilized fracture model of tibia. The mice were sacrific...

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Autores principales: Sang, Xi-Guang, Wang, Zhi-Yong, Cheng, Lin, Liu, Yan-Hong, Li, Yong-Gang, Qin, Tao, Di, Kai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5377403/
https://www.ncbi.nlm.nih.gov/pubmed/28413480
http://dx.doi.org/10.3892/etm.2017.4108
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author Sang, Xi-Guang
Wang, Zhi-Yong
Cheng, Lin
Liu, Yan-Hong
Li, Yong-Gang
Qin, Tao
Di, Kai
author_facet Sang, Xi-Guang
Wang, Zhi-Yong
Cheng, Lin
Liu, Yan-Hong
Li, Yong-Gang
Qin, Tao
Di, Kai
author_sort Sang, Xi-Guang
collection PubMed
description The aim of the present study was to analyze the mechanism by which nerve growth factor (NGF) promotes callus formation in mice with tibial fracture. NGF transgenic homozygotic mice and NGF wild homozygotic mice were selected to construct non-stabilized fracture model of tibia. The mice were sacrificed on days 7, 14 and 21, respectively, and each group had a sample with 8 mice at each point in time. X-ray radiography and safranin fast green were used to observe fracture healing and in situ hybridization was used to examine the NGF mRNA expression of tibia at each phase of fracture healing. Tartrate-resistant acid phosphatase (TRAP) staining of callus tissue and the expression level of TRAP mRNA were combined to observe osteoclast formation. COL2A1, a chondrocyte differentiation-related gene in callus, and the mRNA level of SOX9 were combined to observe chondrocyte differentiation. It was found that under X-ray radiography, the fracture of NGF transgenic homozygotic mice healed in advance (P<0.05). Cartilage and bone tissue were identified by safranin and fast green staining. The residual cartilage on the callus tissue of NGF transgenic homozygotic mice had decreased significantly (P<0.05). The NGF mRNA expression level in each phase of callus formation of NGF transgenic homozygotic mice was significantly higher than that of the wild group (P<0.05). The number of positive cells in NGF-TRAP staining at each time point after fracture and the NGF mRNA expression level was markedly higher than that of the wild group, and the expression levels of COL2A1 and SOX9 mRNA were distinctively higher than that of the wild group. In conclusion, NGF potentially improves the healing of tibial fracture by osteoclast formation. Additionally, an increase in the number of osteoblasts in the NGF transgenic homozygotic mice compared with the wild-type mice may be achieved by cartilage differentiation due to NGF increasing the COL2A1 and SOX9 mRNA expression levels.
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spelling pubmed-53774032017-04-15 Analysis of the mechanism by which nerve growth factor promotes callus formation in mice with tibial fracture Sang, Xi-Guang Wang, Zhi-Yong Cheng, Lin Liu, Yan-Hong Li, Yong-Gang Qin, Tao Di, Kai Exp Ther Med Articles The aim of the present study was to analyze the mechanism by which nerve growth factor (NGF) promotes callus formation in mice with tibial fracture. NGF transgenic homozygotic mice and NGF wild homozygotic mice were selected to construct non-stabilized fracture model of tibia. The mice were sacrificed on days 7, 14 and 21, respectively, and each group had a sample with 8 mice at each point in time. X-ray radiography and safranin fast green were used to observe fracture healing and in situ hybridization was used to examine the NGF mRNA expression of tibia at each phase of fracture healing. Tartrate-resistant acid phosphatase (TRAP) staining of callus tissue and the expression level of TRAP mRNA were combined to observe osteoclast formation. COL2A1, a chondrocyte differentiation-related gene in callus, and the mRNA level of SOX9 were combined to observe chondrocyte differentiation. It was found that under X-ray radiography, the fracture of NGF transgenic homozygotic mice healed in advance (P<0.05). Cartilage and bone tissue were identified by safranin and fast green staining. The residual cartilage on the callus tissue of NGF transgenic homozygotic mice had decreased significantly (P<0.05). The NGF mRNA expression level in each phase of callus formation of NGF transgenic homozygotic mice was significantly higher than that of the wild group (P<0.05). The number of positive cells in NGF-TRAP staining at each time point after fracture and the NGF mRNA expression level was markedly higher than that of the wild group, and the expression levels of COL2A1 and SOX9 mRNA were distinctively higher than that of the wild group. In conclusion, NGF potentially improves the healing of tibial fracture by osteoclast formation. Additionally, an increase in the number of osteoblasts in the NGF transgenic homozygotic mice compared with the wild-type mice may be achieved by cartilage differentiation due to NGF increasing the COL2A1 and SOX9 mRNA expression levels. D.A. Spandidos 2017-04 2017-02-07 /pmc/articles/PMC5377403/ /pubmed/28413480 http://dx.doi.org/10.3892/etm.2017.4108 Text en Copyright: © Sang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Sang, Xi-Guang
Wang, Zhi-Yong
Cheng, Lin
Liu, Yan-Hong
Li, Yong-Gang
Qin, Tao
Di, Kai
Analysis of the mechanism by which nerve growth factor promotes callus formation in mice with tibial fracture
title Analysis of the mechanism by which nerve growth factor promotes callus formation in mice with tibial fracture
title_full Analysis of the mechanism by which nerve growth factor promotes callus formation in mice with tibial fracture
title_fullStr Analysis of the mechanism by which nerve growth factor promotes callus formation in mice with tibial fracture
title_full_unstemmed Analysis of the mechanism by which nerve growth factor promotes callus formation in mice with tibial fracture
title_short Analysis of the mechanism by which nerve growth factor promotes callus formation in mice with tibial fracture
title_sort analysis of the mechanism by which nerve growth factor promotes callus formation in mice with tibial fracture
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5377403/
https://www.ncbi.nlm.nih.gov/pubmed/28413480
http://dx.doi.org/10.3892/etm.2017.4108
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