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In vivo binding of PRDM9 reveals interactions with noncanonical genomic sites
In mouse and human meiosis, DNA double-strand breaks (DSBs) initiate homologous recombination and occur at specific sites called hotspots. The localization of these sites is determined by the sequence-specific DNA binding domain of the PRDM9 histone methyl transferase. Here, we performed an extensiv...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5378176/ https://www.ncbi.nlm.nih.gov/pubmed/28336543 http://dx.doi.org/10.1101/gr.217240.116 |
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author | Grey, Corinne Clément, Julie A.J. Buard, Jérôme Leblanc, Benjamin Gut, Ivo Gut, Marta Duret, Laurent de Massy, Bernard |
author_facet | Grey, Corinne Clément, Julie A.J. Buard, Jérôme Leblanc, Benjamin Gut, Ivo Gut, Marta Duret, Laurent de Massy, Bernard |
author_sort | Grey, Corinne |
collection | PubMed |
description | In mouse and human meiosis, DNA double-strand breaks (DSBs) initiate homologous recombination and occur at specific sites called hotspots. The localization of these sites is determined by the sequence-specific DNA binding domain of the PRDM9 histone methyl transferase. Here, we performed an extensive analysis of PRDM9 binding in mouse spermatocytes. Unexpectedly, we identified a noncanonical recruitment of PRDM9 to sites that lack recombination activity and the PRDM9 binding consensus motif. These sites include gene promoters, where PRDM9 is recruited in a DSB-dependent manner. Another subset reveals DSB-independent interactions between PRDM9 and genomic sites, such as the binding sites for the insulator protein CTCF. We propose that these DSB-independent sites result from interactions between hotspot-bound PRDM9 and genomic sequences located on the chromosome axis. |
format | Online Article Text |
id | pubmed-5378176 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Cold Spring Harbor Laboratory Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-53781762017-04-12 In vivo binding of PRDM9 reveals interactions with noncanonical genomic sites Grey, Corinne Clément, Julie A.J. Buard, Jérôme Leblanc, Benjamin Gut, Ivo Gut, Marta Duret, Laurent de Massy, Bernard Genome Res Research In mouse and human meiosis, DNA double-strand breaks (DSBs) initiate homologous recombination and occur at specific sites called hotspots. The localization of these sites is determined by the sequence-specific DNA binding domain of the PRDM9 histone methyl transferase. Here, we performed an extensive analysis of PRDM9 binding in mouse spermatocytes. Unexpectedly, we identified a noncanonical recruitment of PRDM9 to sites that lack recombination activity and the PRDM9 binding consensus motif. These sites include gene promoters, where PRDM9 is recruited in a DSB-dependent manner. Another subset reveals DSB-independent interactions between PRDM9 and genomic sites, such as the binding sites for the insulator protein CTCF. We propose that these DSB-independent sites result from interactions between hotspot-bound PRDM9 and genomic sequences located on the chromosome axis. Cold Spring Harbor Laboratory Press 2017-04 /pmc/articles/PMC5378176/ /pubmed/28336543 http://dx.doi.org/10.1101/gr.217240.116 Text en © 2017 Grey et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by/4.0/ This article, published in Genome Research, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Research Grey, Corinne Clément, Julie A.J. Buard, Jérôme Leblanc, Benjamin Gut, Ivo Gut, Marta Duret, Laurent de Massy, Bernard In vivo binding of PRDM9 reveals interactions with noncanonical genomic sites |
title | In vivo binding of PRDM9 reveals interactions with noncanonical genomic sites |
title_full | In vivo binding of PRDM9 reveals interactions with noncanonical genomic sites |
title_fullStr | In vivo binding of PRDM9 reveals interactions with noncanonical genomic sites |
title_full_unstemmed | In vivo binding of PRDM9 reveals interactions with noncanonical genomic sites |
title_short | In vivo binding of PRDM9 reveals interactions with noncanonical genomic sites |
title_sort | in vivo binding of prdm9 reveals interactions with noncanonical genomic sites |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5378176/ https://www.ncbi.nlm.nih.gov/pubmed/28336543 http://dx.doi.org/10.1101/gr.217240.116 |
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