Assessing alternative base substitutions at primer CpG sites to optimise unbiased PCR amplification of methylated sequences

BACKGROUND: Determining the role of DNA methylation in various biological processes is dependent on the accurate representation of often highly complex patterns. Accurate representation is dependent on unbiased PCR amplification post bisulfite modification, regardless of methylation status of any gi...

Descripción completa

Detalles Bibliográficos
Autores principales: Candiloro, Ida L. M., Mikeska, Thomas, Dobrovic, Alexander
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5379501/
https://www.ncbi.nlm.nih.gov/pubmed/28392841
http://dx.doi.org/10.1186/s13148-017-0328-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5379501/
https://www.ncbi.nlm.nih.gov/pubmed/28392841
http://dx.doi.org/10.1186/s13148-017-0328-4

Ejemplares similares