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Assessing alternative base substitutions at primer CpG sites to optimise unbiased PCR amplification of methylated sequences
BACKGROUND: Determining the role of DNA methylation in various biological processes is dependent on the accurate representation of often highly complex patterns. Accurate representation is dependent on unbiased PCR amplification post bisulfite modification, regardless of methylation status of any gi...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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BioMed Central
2017
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5379501/ https://www.ncbi.nlm.nih.gov/pubmed/28392841 http://dx.doi.org/10.1186/s13148-017-0328-4 |