Validation of microinjection methods for generating knockout mice by CRISPR/Cas-mediated genome engineering

The CRISPR/Cas system, in which the Cas9 endonuclease and a guide RNA complementary to the target are sufficient for RNA-guided cleavage of the target DNA, is a powerful new approach recently developed for targeted gene disruption in various animal models. However, there is little verification of mi...

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Detalles Bibliográficos
Autores principales: Horii, Takuro, Arai, Yuji, Yamazaki, Miho, Morita, Sumiyo, Kimura, Mika, Itoh, Masahiro, Abe, Yumiko, Hatada, Izuho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5380110/
https://www.ncbi.nlm.nih.gov/pubmed/24675426
http://dx.doi.org/10.1038/srep04513

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5380110/
https://www.ncbi.nlm.nih.gov/pubmed/24675426
http://dx.doi.org/10.1038/srep04513

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