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Validation of microinjection methods for generating knockout mice by CRISPR/Cas-mediated genome engineering
The CRISPR/Cas system, in which the Cas9 endonuclease and a guide RNA complementary to the target are sufficient for RNA-guided cleavage of the target DNA, is a powerful new approach recently developed for targeted gene disruption in various animal models. However, there is little verification of mi...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group
2014
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5380110/ https://www.ncbi.nlm.nih.gov/pubmed/24675426 http://dx.doi.org/10.1038/srep04513 |