Design and application of a lactulose biosensor

In this study the repressor of Escherichia coli lac operon, LacI, has been engineered for altered effector specificity. A LacI saturation mutagenesis library was subjected to Fluorescence Activated Cell Sorting (FACS) dual screening. Mutant LacI-L5 was selected and it is specifically induced by lact...

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Detalles Bibliográficos
Autores principales: Wu, Jieyuan, Jiang, Peixia, Chen, Wei, Xiong, Dandan, Huang, Linglan, Jia, Junying, Chen, Yuanyuan, Jin, Jian-Ming, Tang, Shuang-Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5384092/
https://www.ncbi.nlm.nih.gov/pubmed/28387245
http://dx.doi.org/10.1038/srep45994

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5384092/
https://www.ncbi.nlm.nih.gov/pubmed/28387245
http://dx.doi.org/10.1038/srep45994

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