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Design and application of a lactulose biosensor
In this study the repressor of Escherichia coli lac operon, LacI, has been engineered for altered effector specificity. A LacI saturation mutagenesis library was subjected to Fluorescence Activated Cell Sorting (FACS) dual screening. Mutant LacI-L5 was selected and it is specifically induced by lact...
Autores principales: | Wu, Jieyuan, Jiang, Peixia, Chen, Wei, Xiong, Dandan, Huang, Linglan, Jia, Junying, Chen, Yuanyuan, Jin, Jian-Ming, Tang, Shuang-Yan |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5384092/ https://www.ncbi.nlm.nih.gov/pubmed/28387245 http://dx.doi.org/10.1038/srep45994 |
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