Cargando…

Expression, Functional Characterization, and Solid-State NMR Investigation of the G Protein-Coupled GHS Receptor in Bilayer Membranes

The expression, functional reconstitution and first NMR characterization of the human growth hormone secretagogue (GHS) receptor reconstituted into either DMPC or POPC membranes is described. The receptor was expressed in E. coli. refolded, and reconstituted into bilayer membranes. The molecule was...

Descripción completa

Detalles Bibliográficos
Autores principales:	Schrottke, Stefanie, Kaiser, Anette, Vortmeier, Gerrit, Els-Heindl, Sylvia, Worm, Dennis, Bosse, Mathias, Schmidt, Peter, Scheidt, Holger A., Beck-Sickinger, Annette G., Huster, Daniel
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2017
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5384189/ https://www.ncbi.nlm.nih.gov/pubmed/28387359 http://dx.doi.org/10.1038/srep46128

Ejemplares similares

Integrating Solid-State NMR and Computational Modeling to Investigate the Structure and Dynamics of Membrane-Associated Ghrelin
por: Vortmeier, Gerrit, et al.
Publicado: (2015)

The Dynamics of the Neuropeptide Y Receptor Type 1 Investigated by Solid-State NMR and Molecular Dynamics Simulation
por: Vogel, Alexander, et al.
Publicado: (2020)

Automated solid-phase peptide synthesis to obtain therapeutic peptides
por: Mäde, Veronika, et al.
Publicado: (2014)

Improved in Vitro Folding of the Y(2) G Protein-Coupled Receptor into Bicelles
por: Schmidt, Peter, et al.
Publicado: (2018)

The Conformational Equilibrium of the Neuropeptide Y2 Receptor in Bilayer Membranes
por: Krug, Ulrike, et al.
Publicado: (2020)

Peptide-Drug Conjugates and Their Targets in Advanced Cancer Therapies
por: Hoppenz, Paul, et al.
Publicado: (2020)

Integrating GHS into the Ghrelin System
por: Veldhuis, Johannes D., et al.
Publicado: (2010)

Unusually persistent Gα(i)-signaling of the neuropeptide Y(2) receptor depletes cellular G(i/o) pools and leads to a G(i)-refractory state
por: Ziffert, Isabelle, et al.
Publicado: (2020)

Integration of Cell-Free Expression and Solid-State NMR to Investigate the Dynamic Properties of Different Sites of the Growth Hormone Secretagogue Receptor
por: Pacull, Emelyne M., et al.
Publicado: (2020)

Development of Fluorinated Non-Peptidic Ghrelin Receptor Ligands for Potential Use in Molecular Imaging
por: Moldovan, Rareş-Petru, et al.
Publicado: (2017)

Cyclic Derivatives of the Chemerin C-Terminus as Metabolically Stable Agonists at the Chemokine-like Receptor 1 for Cancer Treatment
por: Fischer, Tobias F., et al.
Publicado: (2021)

Cell-Free Expression and Photo-Crosslinking of the Human Neuropeptide Y(2) Receptor
por: Kögler, Lisa Maria, et al.
Publicado: (2019)

Shuttling of Peptide‐Drug Conjugates by G Protein‐Coupled Receptors Is Significantly Improved by Pulsed Application
por: Ziffert, Isabelle, et al.
Publicado: (2020)

Development of a ghrelin receptor inverse agonist for positron emission tomography
por: Bergmann, Ralf, et al.
Publicado: (2021)

β Cell GHS-R Regulates Insulin Secretion and Sensitivity
por: Pradhan, Geetali, et al.
Publicado: (2021)

Laboratory chemical waste: hazard classification by GHS and transport risk
por: Giovanni, Cássio, et al.
Publicado: (2021)

The Structural Basis of Peptide Binding at Class A G Protein-Coupled Receptors
por: Vu, Oanh, et al.
Publicado: (2021)

GHS-R1a constitutive activity and its physiological relevance
por: Mear, Yves, et al.
Publicado: (2013)

Complexes of Ghrelin GHS-R1a, GHS-R1b, and Dopamine D(1) Receptors Localized in the Ventral Tegmental Area as Main Mediators of the Dopaminergic Effects of Ghrelin
por: Navarro, Gemma, et al.
Publicado: (2022)

The Role of Ghrelin/GHS-R1A Signaling in Nonalcohol Drug Addictions
por: Sustkova-Fiserova, Magdalena, et al.
Publicado: (2022)

Probing the Y(2) Receptor on Transmembrane, Intra- and Extra-Cellular Sites for EPR Measurements
por: Laugwitz, Jeannette M., et al.
Publicado: (2020)

Formation of the β-barrel assembly machinery complex in lipid bilayers as seen by solid-state NMR
por: Pinto, Cecilia, et al.
Publicado: (2018)

The Expression of GHS-R in Primary Neurons Is Dependent upon Maturation Stage and Regional Localization
por: Lattuada, Donatella, et al.
Publicado: (2013)

Obestatin stimulates glucose-induced insulin secretion through ghrelin receptor GHS-R
por: Pradhan, Geetali, et al.
Publicado: (2017)

Analysing COVID-19 outcomes in the context of the 2019 Global Health Security (GHS) Index
por: Rose, Sophie M, et al.
Publicado: (2021)

Neuronal GHS-R Differentially Modulates Feeding Patterns under Normal and Obesogenic Conditions
por: Lee, Jong Han, et al.
Publicado: (2022)

Mechanistic Investigation of GHS-R Mediated Glucose-Stimulated Insulin Secretion in Pancreatic Islets
por: Pradhan, Geetali, et al.
Publicado: (2022)

Much higher prevalence of keratoconus than announced results of the Gutenberg Health Study (GHS)
por: Marx-Gross, Susanne, et al.
Publicado: (2023)

Reconstitution and NMR Characterization of the Ion-Channel Accessory Subunit Barttin in Detergents and Lipid-Bilayer Nanodiscs
por: Viennet, Thibault, et al.
Publicado: (2019)

Fibrils of Truncated Pyroglutamyl-Modified Aβ Peptide Exhibit a Similar Structure as Wildtype Mature Aβ Fibrils
por: Scheidt, Holger A., et al.
Publicado: (2016)

The Potential of α-Spinasterol to Mimic the Membrane Properties of Natural Cholesterol
por: Haralampiev, Ivan, et al.
Publicado: (2017)

Effects of the RNA-Polymerase Inhibitors Remdesivir and Favipiravir on the Structure of Lipid Bilayers—An MD Study
por: Bringas, Mauro, et al.
Publicado: (2022)

Suppression of GHS-R in AgRP Neurons Mitigates Diet-Induced Obesity by Activating Thermogenesis
por: Wu, Chia-Shan, et al.
Publicado: (2017)

Does H(2) Alter Mitochondrial Bioenergetics via GHS-R1α Activation?
por: Ostojic, Sergej M.
Publicado: (2017)

GHS-R in brown fat potentiates differential thermogenic responses under metabolic and thermal stresses
por: Lee, Jong Han, et al.
Publicado: (2021)

Expression of the growth hormone secretagogue receptor 1a (GHS‐R1a) in the brain
por: Airapetov, Marat I., et al.
Publicado: (2021)

A Chiral (19)F NMR Reporter of Foldamer Conformation in Bilayers
por: Wang, Siyuan, et al.
Publicado: (2022)

Structural basis of ligand binding modes at the neuropeptide Y Y(1) receptor
por: Yang, Zhenlin, et al.
Publicado: (2018)

Membrane properties of hydroxycholesterols related to the brain cholesterol metabolism
por: Hilsch, Malte, et al.
Publicado: (2017)

Membrane Interaction of Ibuprofen with Cholesterol-Containing Lipid Membranes
por: Kremkow, Jan, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_qd3p0iiqhh2rpatgvt81hin4n5