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fM to aM nucleic acid amplification for molecular diagnostics in a non-stick-coated metal microfluidic bioreactor

A sensitive DNA isothermal amplification method for the detection of DNA at fM to aM concentrations for pathogen identification was developed using a non-stick-coated metal microfluidic bioreactor. A portable confocal optical detector was utilized to monitor the DNA amplification in micro- to nanoli...

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Detalles Bibliográficos
Autores principales: Huang, Guoliang, Huang, Qin, Ma, Li, Luo, Xianbo, Pang, Biao, Zhang, Zhixin, Wang, Ruliang, Zhang, Junqi, Li, Qi, Fu, Rongxin, Ye, Jiancheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5384283/
https://www.ncbi.nlm.nih.gov/pubmed/25475544
http://dx.doi.org/10.1038/srep07344