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High level expression of recombinant human growth hormone in Escherichia coli: crucial role of translation initiation region
For high-throughput production of recombinant protein in Escherichia coli (E. coli), besides important parameters such as efficient vector with strong promoter and compatible host, other important issues including codon usage, rare codons, and GC content specially at N-terminal region should be cons...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5385732/ https://www.ncbi.nlm.nih.gov/pubmed/28515770 http://dx.doi.org/10.4103/1735-5362.202462 |