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High level expression of recombinant human growth hormone in Escherichia coli: crucial role of translation initiation region

For high-throughput production of recombinant protein in Escherichia coli (E. coli), besides important parameters such as efficient vector with strong promoter and compatible host, other important issues including codon usage, rare codons, and GC content specially at N-terminal region should be cons...

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Detalles Bibliográficos
Autores principales: Ghavim, Mahsa, Abnous, Khalil, Arasteh, Fatemeh, Taghavi, Sahar, Nabavinia, Maryam Sadat, Alibolandi, Mona, Ramezani, Mohammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5385732/
https://www.ncbi.nlm.nih.gov/pubmed/28515770
http://dx.doi.org/10.4103/1735-5362.202462