Practical method for targeted disruption of cilia-related genes by using CRISPR/Cas9-mediated, homology-independent knock-in system

The CRISPR/Cas9 system has revolutionized genome editing in virtually all organisms. Although the CRISPR/Cas9 system enables the targeted cleavage of genomic DNA, its use for gene knock-in remains challenging because levels of homologous recombination activity vary among various cells. In contrast,...

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Detalles Bibliográficos
Autores principales: Katoh, Yohei, Michisaka, Saki, Nozaki, Shohei, Funabashi, Teruki, Hirano, Tomoaki, Takei, Ryota, Nakayama, Kazuhisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2017
Materias:
Brief Reports
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5385939/
https://www.ncbi.nlm.nih.gov/pubmed/28179459
http://dx.doi.org/10.1091/mbc.E17-01-0051

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5385939/
https://www.ncbi.nlm.nih.gov/pubmed/28179459
http://dx.doi.org/10.1091/mbc.E17-01-0051

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