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Challenges of CRISPR/Cas9 applications for long non-coding RNA genes

The CRISPR/Cas9 system provides a revolutionary genome editing tool for all areas of molecular biology. In long non-coding RNA (lncRNA) research, the Cas9 nuclease can delete lncRNA genes or introduce RNA-destabilizing elements into their locus. The nuclease-deficient dCas9 mutant retains its RNA-de...

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Detalles Bibliográficos
Autores principales:	Goyal, Ashish, Myacheva, Ksenia, Groß, Matthias, Klingenberg, Marcel, Duran Arqué, Berta, Diederichs, Sven
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2017
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5388423/ https://www.ncbi.nlm.nih.gov/pubmed/28180319 http://dx.doi.org/10.1093/nar/gkw883

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