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A nanobody-based toolset to investigate the role of protein localization and dispersal in Drosophila

The role of protein localization along the apical-basal axis of polarized cells is difficult to investigate in vivo, partially due to lack of suitable tools. Here, we present the GrabFP system, a collection of four nanobody-based GFP-traps that localize to defined positions along the apical-basal ax...

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Detalles Bibliográficos
Autores principales: Harmansa, Stefan, Alborelli, Ilaria, Bieli, Dimitri, Caussinus, Emmanuel, Affolter, Markus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5388529/
https://www.ncbi.nlm.nih.gov/pubmed/28395731
http://dx.doi.org/10.7554/eLife.22549