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Duplication-Insertion Recombineering: a fast and scar-free method for efficient transfer of multiple mutations in bacteria
We have developed a new λ Red recombineering methodology for generating transient selection markers that can be used to transfer mutations between bacterial strains of both Escherichia coli and Salmonella enterica. The method is fast, simple and allows for the construction of strains with several mu...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2017
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5389514/ https://www.ncbi.nlm.nih.gov/pubmed/27899661 http://dx.doi.org/10.1093/nar/gkw1078 |